Establishment of seasonal influenza vaccine strain development platform
10.3760/cma.j.issn.1003-9279.2015.02.027
- VernacularTitle:季节性流感疫苗株制备技术平台的建立
- Author:
Li XIN
1
;
Jian LU
;
Yongkun CHEN
;
Jing TANG
;
Junfeng GUO
;
Dayan WANG
;
Yuelong SHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Influenza A virus;
Reassortment viruses;
Influenza;
Influenza vaccine
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):174-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to establish classical reassortment platform to produce seasonal influenza vaccine strain in China.Methods A representative wild type influenza A H1N1 virus (A/Sichuan/1/2009) was selected to reassort with an egg adapted donator X157.Three sera suppressions were performed to select egg adapted high yield candidate vaccine strain.Results The reassortant virus was characterized and the result showed that two surface proteins were from wild type virus and internal genes M and NP were from donor.The HA titer was 1024.The reassortant was antigenically similar with wild type virus.Conclusion The reassortant is an egg adapt high yield virus.The establishment of the classical reassortment platform lays the foundation for developing seasonal influenza vaccine viruses in China.
