Recombinant humanized antibodies against rabies virus phosphoprotein (RVP)
10.3760/cma.j.issn.1003-9279.2015.02.025
- VernacularTitle:人源抗狂犬病毒P蛋白基因工程抗体的研究
- Author:
Yang LIU
1
;
Lina SUN
;
Chuan LI
;
Mifang LIANG
;
Dexin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
- Keywords:
Rabies virus;
Bacterioghages;
Antibodies,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):168-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain recombinant human anti-RVP genetically engineered antibodies from rabiator-derived antibody phage library.Metbods A combinatorial human Fab library against rabies virus was constructed with antibody genes harvested from peripheral blood of rabiators with high titer of antibody.The library was panned with phage display and selected by purified CTN-1 virus particles.After that the selected antibody was converted to full human IgG antibody with recombinant mammal cell antibodyexpression system.Results Unique 1 human Fab antibodies specific for RVP were obtained and conformed by competitive ELISA,Western Blotting and IFA.IFA,competitive ELISA and Western blotting showed the panned antibody 1A2 bound specifically to RVP.Conclusion Human antibody against RVP was firstly obtained through phage antibody library,and could be used for RVP functional mechanism research and rabies diagnosis.
