Experimental study on the different day-night expression of hypothalamus SCN Clock mRNA in mice under different light systems and Wendan Tang's intervention
10.3969/j.issn.1006-2157.2017.08.005
- VernacularTitle:不同光制下小鼠下丘脑视交叉上核Clock mRNA昼夜表达特点及温胆汤的干预作用
- Author:
Yang YANG
1
;
Mingquan ZHANG
;
Xiaotian PU
;
Dongyu TAN
;
Shuran MA
Author Information
1. 河北中医学院基础医学院 河北 050200
- Keywords:
wendan tang;
melatonin;
biological clock;
gene;
light;
correspondence between man and universe;
mouse
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(8):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the characteristics of the hypothalamus SCN Clock mRNA expression in mice under four timing points in a day and light condition and the effects of Wendan Tang (Gallbladder-Warming Decoction,WDT).Methods 160 male KMmice of 2 ～3 months were randomly divided into normal group (6:00—18:00 light),dark group (24 h dark),model group (24 h light),WDT group, and melatonin group(n =32 in each group).Zeitgeber time (ZT)and Circadian time (CT)were used to indicate natural light and dark timing points respectively At the last day of the 12 th week,8 mice from each group were randomly selected and sacrificed at natural light timing points of 6 a.m.(ZT0 /CT0), 12 (ZT6 /CT6),18 (ZT12 /CT12),and 24 (ZT18 /CT18).The concentration of melatonin in ser-um was measured by using enzyme-linked immunosorbent assay (ELISA).The expression of SCN Clock mRNA in mouse hypothalamus was detected by using polymerase chain reaction (PCR) method. Results Serum melatonin:The melatonin level at ZT18 of mice in the normal group was the highest (P<0.05)among all groups.Serum melatonin level in mice of the dark group at CT0 was lower than those at CT18 and CT12 (P <0.05).There were no differences in the melatonin levels between four timing points in mice of the model group (P >0.05).Serum melatonin levels in mice of the WDT group were lower at ZT0 and ZT6 than that at ZT18 (P <0.05)while these values at ZT0,ZT6 and ZT12 were lower than that at ZT18 in mice of the melatonin group (P <0.05).Expression of SCN Clock mRNA in mice hypothalamus:The expression of SCN Clock mRNA was lower at ZT18 than that at ZT6 (P <0.05).No differences were found at the four timing points in mice of the dark group (P >0.05).The expression levels in mice of the model group were lower at ZT0 and ZT12 compared with ZT18 (P <0.05);also lower at ZT0 and ZT12 compared with ZT 6 (P <0.05).The expression levels in mice of WDT and me-latonin groups at ZT18 were lower than those at ZT6 (P <0.05).Conclusion WDT is rich in melato-nin and could adjust biological clock rhythm disorder and reduction of melatonin due to continuous light.
