Characteristics of EBER gene of Epstein-Barr virus in EBV infected children in Beijing area
10.3760/cma.j.issn.1003-9279.2015.02.010
- VernacularTitle:北京地区儿童EBV感染疾病中EBER基因变异特征分析
- Author:
Weifeng LU
1
;
Junhong AI
;
Yali LIU
;
Chunyan LIU
;
Zhengde XIE
Author Information
1. 100045北京,首都医科大学附属北京儿童医院、北京市儿科研究所,儿科学国家重点学科,省部共建儿科重大疾病研究重点实验室
- Keywords:
Herpesvirus 4,human;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):124-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristic of EBER gene of Epstein—Barr virus in EBV infection in children in Beijing area.Methods Total DNA was extracted from whole blood of 288 patents with EBV-associated infectious mononucleosis (IM),52 patients with EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH) and 9 patients with chronic active Epstein-Barr virus (CAEBV) infection in Beijing Children' s Hospital.The subtype of EBV type Ⅰ / Ⅱ was defined by the size of amplicon of the EBNA3C genes.The amplified EBER gene products were sequenced directly and the sequences were analyzed by BioEdit 7.0.9 and MEGA 4.0.2.The relationship between EBV subtype or EBER genotype and clinical phenotype were statistically analyzed.Results Type Ⅰ EBV was present in 98.6％ samples (344/ 349) and type Ⅱ EBV in 1.4％ samples (5/349).Three EBER genotypes,including EB-6m,EB-8m and EB-7m,were defined in this study.Thc detection rates of EB-6m,EB-8m and EB-7m were 96％,2.9％ and 1.1％ respectively.There were no significant differences in the frequencies of the three genotypes among IM,EBV-HLH and CAEBV (P ＞ 0.05).Conclusion EBV type Ⅰ is predominant in EBV infection in children in Beijing area.EB-6m genotype was predominant genotype in this area.
