Specific immune responses of recombinant plasmid pVR-S and recombinant adenovirus rAdV-S
10.3760/cma.j.issn.1003-9279.2015.02.008
- VernacularTitle:重组质粒pVR-S与重组腺病毒rAdV-S诱导特异性免疫反应的研究
- Author:
Yinghui WANG
1
;
Guomin CHEN
;
Shengli BI
;
Yi ZENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatiis B surface antigens;
DNA;
Adenoviridae;
Immunization,secondary
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):118-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to analyze the immune effects of pVR-S and rAdV-S with different immune strategies.Methods HBsAg gene was used to construct recombinant DNA plasmid named pVR-S and recombinant adenovirus named rAdV-S,respectively.Firstly,BALB/c mice were injected with pVR-S by two methods,including electroporation and intramuscular injection.Secondly,two groups of mice were immunized with rAdV-S and pVR-S,respectively.Level of HBsAb was detected by ELISA and cellular responses were detected by Elispot to evaluate the immune effects.Finally,DNA primeAdenovirus boost strategy was used to evaluate the humoral and cellular immune responses.Results Compared with intramuscular immunization,electroporation-mediated immunization could induce stronger humoral immune response.Independent pVR-S increased HBsAb level slowly,while independent rAdV-S induced HBsAb at week 2 with higher HBsAb level than pVR-S.What is more,pVR-S prime-rAdV-S boost strategy could result in two to three times higher cellular responses than independent immunizations.Conclusion Recombinant adenovirus rAdV-S could induce strong humoral and cellular immune responses.pVR-S prime-rAdV-S boost immunization was better than independent rAdV-S immunization,contributing to excellent antiviral capability.
