Effect and mechanism of Bone Marrow Mesenchymal Stem Cells on autologous lymphocyte proliferation in patients with decompensated liver cirrhosis
10.3760/cma.j.issn.1003-9279.2015.02.002
- VernacularTitle:失代偿性肝硬化患者骨髓间充质干细胞对自体淋巴细胞增殖的影响及机制研究
- Author:
Chunhui GUO
1
;
Lanxiu HAN
;
Meirong WAN
;
Guojiong DENG
;
Jianhe GAN
Author Information
1. 苏州大学附属第一医院
- Keywords:
Cirrhosis;
Stem cells;
Lymphocyte;
Interleukin-10
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):100-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of bone marrow mesenchymal stem cells (BMSCs) on autologous peripheral blood lymphocyte proliferation in vitro in patients with hepatitis B virus related decompensated cirrhosis.Methods MSCs were isolated and expanded from human bone marrow blood of nine patients with decompensated cirrhosis,four groups were designed for experiment:①BMSCs + lymphocytes + PHA (contact co-culture) ; ② BMSCs + lymphocytes + PHA (non-contact co-culture) ; ③lymphocytes + PHA (positive control) ; ④lymphocytes alone (negative control).Lymphocytes proliferation rate were detected by flow cytometry.The mRNA expression levels of interleukin-10 (IL-10)and transforming growth factor-β1 (TGF-β1)in BMSCs were detected by using reverse transcription-polymerase chain reaction (RT-PCR).Results Cell proliferation of contact and non-contact co-culture groups significantly declined when compared with that of positive control group (all P ＜ 0.01),The relative expression levels of IL-10 mRNA and TGF-βlmRNA in BMSCs of contact and non-contact co-culture group raised up significantly after culture (all P ＜ 0.01).Besides,there was no significant difference on lymphocyte proliferation rate or expression levels of IL-10mRNA and TGF-β1 mRNA between contact co-culture group and non-contact coculture group (all P ＞ 0.05).Conclusion BMSCs from cirrhotic patients can inhibit proliferation of autologous peripheral blood lymphocytes,the mechanism may be associated with the secretion of inhibitory cytokine IL-10 and TGF-β1 in BMSCs.
