Adaptation of seasonal influenza virus H1N1 and H3N2 vaccine strains in BALB/c mouse pneumonitis model
10.3760/cma.j.issn.1003-9279.2015.02.001
- VernacularTitle:季节性流感病毒H1N1和H3N2疫苗株在BALB/c小鼠中的传代适应
- Author:
Baoying HUANG
1
;
Xiuping WANG
;
Wenling WANG
;
Wenjie TAN
;
Li RUAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 冠状病毒和痘病毒相关疾病室
- Keywords:
Influenza A virus;
Influenza vaccine;
Mice,inbred BALB C
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(2):97-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of adaptation seasonal influenza virus H1N1 and H3N2 vaccine strains in BALB/C mice.Methods BALB/c mice were infected with wild-type virus and lungs were dissected out at peak of virus multiplication to generate the first passage of mouse adapted virus (P1),which was subjected serial mouse lung-to-lung passages (from P1 to P10).Pathogenicity and genome sequences of wide-type and mouse-adapted virus were compared.Results For H3N2 vaccine strain,virus titer was under detection at any passages.In contrast,for H1N1 vaccine strain,virus replication ability and pathogenicity increased by lung-to-lung passage.Genome sequence analysis of H1Nlwt and H1N1MA indicated that mutation of HA-N142D contributed to the enhanced virulence.Conclusions H3N2 vaccine strain was difficult to replicate in mice while H1 N1 vaccine strain could effectively replicate in BALB/c mice and mutation of HA-N142D contributed to the enhanced virulence.
