Effects of Huangqi and Ezhu combined with oxaliplatin on CXCR3 and CCR6 expression in mice with CT26.WT orthotopically transplanted tumor
10.3969/j.issn.1006-2157.2017.06.006
- VernacularTitle:黄芪、莪术配伍联合奥沙利铂对CT26.WT原位移植瘤小鼠中CXCR3、CCR6表达影响
- Author:
Zifeng CAO
1
;
Decai TANG
;
Xiaoxia SHI
;
Suyu YANG
Author Information
1. 南京中医药大学 江苏 210023
- Keywords:
CXCR3;
CCR6;
liver metastasis;
colorectal cancer;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(6):477-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of extract from couple medicinals of strengthening healthy qi and eliminating pathogenic qi, i.e.Huangqi(Radix Astragali, astragalus root) and Ezhu (Rhizoma Curcumae, curcumae rhizome), and oxaliplatin(L-OHP)on inhibiting metastasis of CT26 wild-type(CT26.WT)cells, and regulating CXCR3, CCR6 mRNA, and protein expression in rats with colon cancer.Methods Orthotopic transplantation model of BALB/c colon carcinoma was built in mice.L-OHP or L-OHP combined with extract from huangqi and ezhu were given to the mice model.Effects of those medicines on liver metastasis were observed.CXCR3 and CCR6 mRNA expression were measured by using RT-PCR, and CXCR3, CCR6 protein expression was detected with Western Blot.Results L-OHP group and L-OHP combined with Chinese medicinal groups exhibited inhibitive effects on orthotropic tumor growth, and best effect was observed in positive expression (P<0.01).L-OHP combined with TCM can decrease the incidence of liver metastasis (P<0.05).Compared with the control group, mRNA expressions of CXCR3 and CCR6 decreased in the model group and low-dose group (P<0.01).Effects of L-OHP combined with low-dose Chinese medicine were more effective than other groups(P<0.01).Compared with the control group, CXCR3 and CCR6 protein expressions decreased sinificantly in all groups(P<0.05 or P<0.01).Conclusion Mechanism of huangqi, ezhu and oxaliplatin(L-OHP) to inhibit tumor metastasis may relate to downregulation of CXCR3, CCR6 mRNA and protein expression.
