Effects of Wuzi Yanzong Wan on improving heat stress resistance of sertoli cell in rats based on gene expression profile
10.3969/j.issn.1006-2157.2017.06.005
- VernacularTitle:五子衍宗丸提高大鼠睾丸支持细胞抵抗热应激能力的基因表达谱分析
- Author:
Yan CAO
1
;
Junyun ZHAO
;
Jian GUO
;
Yue LI
;
Suqin HU
;
Xuejiao JIANGUO
Author Information
1. 北京中医药大学 北京 100029
- Keywords:
Wuzi Yanzong pill;
gene expression profile;
sertoli cells;
heat stress resistance;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(6):471-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of Wuzi Yanzong Wan (Five-Seed Progeny Pill) on improving heat stress resistance of sertoli cells in rats based on data from the whole genome microarray.Methods Sertoli cells in primary culture were randomly divided into Wuzi Yanzong Wan (WZYZW) group, normal group and model group.The cells in three groups were given WZYZW solution and equal volume of PBS respectively.After 24 hours, rats in WZYZW group and model group were placed in water bath at 43 ℃ for 15 minutes, while rats in normal group were put in water bath at 37 ℃ for 15 minutes, then total RNA of three groups were extracted respectively and data was analyzed with gene chip technology.Results Compared with the model group, there were totally 1,203 differently genes in WZYZW group based on Sertoli cell gene expression profiles, in which 697 were down regulated and 506 were up-regulated.There were totally 473 differently expressed genes in the normal group, including 168 up-regulated genes and 305 down regulated genes.Among these down-regulated gene expressions, RGD1305298, Trpt1, Dll3, Abi3 and Ppp1r12b were significant in the WZYZW group and normal group, while Dusp7 was significant among up-regulated gene expressions.The genes mentioned above were related to cell cycle, apoptosis, cell metabolism, cell adhesion and Notch signal pathway, regulation of actin cytoskeleton, MAPK signaling pathway and tight junction pathway, etc.Conclusion The mechanism of Wuzi Yanzong pill in improving heat stress resistance of sertoli cells may involve maintenance of tight junctions between sertoli cells, inhibition of sertoli cells apoptosis and promotion of cell differentiation and maturation.
