Study of hepatitis C virus core antigen detection in voluntary blood donation
10.3760/cma.j.issn.1003-9279.2015.01.023
- VernacularTitle:丙型肝炎病毒核心抗原检测在无偿献血中的应用探讨
- Author:
Hao LYU
1
;
Sujun JIANG
;
Aihua SUN
Author Information
1. 浙江省衢州市中心血站
- Keywords:
Hepatitis C;
Blood donor;
Hepacivirus
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(1):68-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect anti-hepatitis C virus enzyme-linked immunoassay (anti-HCV ELISA) positive blood specimens by HCV core antigen ELISA(HCV-cAgELISA),and to investigate the value of detecting of HCV-cAg for screening blood donor.Methods All anti-HCV ELISA positive specimens were identified by using HCV-cAgELISA and HCV RT-PCR methods.Results In 225 anti-HCV positive specimens,the positive rates of HCV-cAgELISA and HCV RT-PCR were 39.1％ and 38.7％,respectively,the positive rates were no significant difference.Among anti-HCV screening unqualified samples,the false positive rates of anti-HCV ELISA in both positive,single positive,and grey area were 31.4％,90.4％ and 83.0％,respectively.The total false positive rate was 61.3％.The false positive rate of HCV-cAgELISA in both positive,single positive,and grey area were 1.4％,0 and 0,respectively.The total false positive rate was 1.1％.The false positive rate of HCV-cAgELISA was significantly lower antiHCV ELISA (P ＜ 0.01).Conclusion Sensitivity of HCVcAg ELISA is silllilar to HCV RT-PCR (P ＞ 0.05).The total false positive rate of HCVcAg ELISA was significantly lower anti-HCV ELISA (P ＜ 0.01).HCVcAg ELISA is a simple,fast,and reliable memod to screen donor with HCV infection in blood transfusion medicine.
