The eEffects of Pixu Yihao Fang on small intestinal GLUT1 of small intestine in spleen-deficient rats with functional dyspepsia rats with spleen deficiency
10.3969/j.issn.1006-2157.2017.06.003
- VernacularTitle:脾虚1号方对脾虚证功能性消化不良大鼠小肠葡萄糖转运蛋白1的影响
- Author:
Lin LYU
1
;
Fengyun WANG
;
Xudong TANG
;
Xiangxue MA
;
Xiaolan YIN
;
Xiaoshuang SHI
Author Information
1. 中国中医科学院西苑医院消化科 北京市中医脾胃病研究所 北京 100091
- Keywords:
Pixu Yihao Fang;
pattern of spleen deficiency;
functional dyspepsia;
glucose transporter Ⅰ;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(6):456-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Pixu Yihao Fang (PXYHF, No.1 Formula for Spleen Deficiency) on the expression of Glucose Transporter (GLUT1)protein (GLUT1) and mRNA in small intestine tissue of FD spleen-deficient rats and functional dyspepsia (FD) the intervention of Pixu I Fang.Methods 70 10-day-old male SD rat pups were randomly divided into normal control group (n=10), FD model group (single model, n=10), and spleen-deficient FD model group (double model, n=50).The normal control group received intragastric administration of 0.2 mL of 2% sucrose, while the single and double model groups were given 0.2 mL of 0.1 % iodoacetamide (IA)IA in 2 % sucrose, for 6 days.6 weeks after birth consecutive days, the Modified multiple platform method (MMPM) was also applied to establish double model for 14 days after week 6.These spleen-deficient FD rats were randomly divided into double model control group, domperidone group, PXYHF low-dose, medium-dose and high-dose dose groups, and were orally gavaged with 10 mL/(kg·d) of distilled water, 3.125 mg/(kg·d) of domperidone, 1.275, 2.55, 5.1 g/(kg·d) of PXYHF respectively for 14 days.Immunohistochemistry, Western-blot and RT-PCR methods were used to measure the GLUT1 protein expression in liver tissues.Results Compared with the normal group, average optical density of GLUT1 protein in small intestinal mucosal tissue, the expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of double model group were decreased (P<0.05, P<0.01);Compared with double model group, average optical density of GLUT1 protein in small intestinal mucosa tissue, expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of low-dose PXYHF were increased (P<0.01).Conclusion The expression of GLUT1 gene and protein decreased in the FD rats with spleen deficiency, and Pixu Yihao Fang could increase the expression of GLUT1 gene and protein.
