Characteristics analysis on major encoded genes VP7, VP4, VP6 and NSP4 of human G9P 6 rotavirus LL51695

VernacularTitle:我国人罕见G9P[6]基因型A组轮状病毒LL51695株VP7、VP4、VP6和NSP4编码基因的分子特征
Author: Dandi LI ¹ ; Xiangyu KONG ; Xin MA ; Jianhui GUO ; Qiuli YU ; Zhaojun DUAN
Author Information

1. 102206,中国疾病预防控制中心病毒病预防控制所
Keywords: Rotavirus; Diarrhea; Swine
From: Chinese Journal of Experimental and Clinical Virology 2015;29(1):59-61
CountryChina
Language:Chinese
Abstract: Objective To explore the evolutionary and molecular characteristics of rare RVA genetype G9P[6] (LL51695) isolated in our laboratory.Methods Major genome segments of RVA strain LL51695 was amplified by reverse transcription-polymerase chain reaction (RT-PCR),and the segments were sequenced.The genome segments of LL51695 was determined by the online RotaC genotyping tool (RotaC v2.0).The reference sequences of every genome segments of RVA was downloaded from GenBank.Homology and phylogenetic evolutionary analyses were conducted using the MEGA 5.0 and DNAStar software package.Results LL51695 isolated from human in Lulong is a rare genotype constellation:G9-P[6]-I5-E1.Conclusion The VP7,VP4,VP6,NSP4 genes were all porcine origins,the strain may be porcine rotaviruses direct infect human.