Regulating effect of Zuogui Jiangtang Jieyu Fang on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in simulated condition of diabetes complicating depression
10.3969/j.issn.1006-2157.2017.05.007
- VernacularTitle:左归降糖解郁方对糖尿病并发抑郁症海马神经元CaMKⅡ及SynGap的调控作用
- Author:
Jian LIU
1
;
Yuhong WANG
;
Yuanshan HAN
;
Yalan XU
;
Lin LIU
;
Hui YANG
;
Wei LI
;
Yun XIANG
Author Information
1. 湖南中医药大学第一附属医院 湖南 410007
- Keywords:
diabetes complicating depression;
cell culture;
hippocampus neurons;
Zuogui Jiangtang Jieyu Fang;
calmodulin dependent protein kinase II;
SynGap;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(5):392-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Zuogui Jiangtang Jieyu Fang (ZJJF) on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in rats simulated diabetes complicating depression DD.Methods The hippocampus neurons were primitively isolated, purified and cultured in SD rats.The DD model was established by applying intervention of high glucose and cortisone.The cultured hippocampus neurons were randomly divided into normal group, model group, medicated serum of ZJJF (ZJJF group), medicated serum of positive drug (fluoxetine and metform) group (positive drug group) and blank group.The damages of hippocampus neurons were detected by using Nissl's staining in all groups, and expressions of CaMKII and SynGap were detected by using immunofluorescence staining and high-content analysis (HCA) after drug intervervention for 18 d.Results Compared with normal group and blank group, the quantity of Nissl bodies decreased significantly, neural network and dentritic spines ruptured or disappeared, and expressions of CaMKII and SynGap were significantly down-regulated in model group (P<0.01).Compared with model group, the quantity of Nissl bodies, neural network and dentritic spines, and synaptic connection were significantly recovered, and expressions of CaMKII and SynGap were un-regulated in positive drug group and ZJJF group (P<0.01 or P<0.05).Conclusion ZJJF had significant regulative effect on CaMKII and SynGap in simulated condition of diabetes complicating depression, which may be one of important mechanisms of ZJJF antagonizing cellular damage and neural degeneration.
