Altered expression of peroxiredoxin 3 in cervical epitheliums transfected with human papillomavirus type 16

VernacularTitle:过氧化物还原酶3在宫颈上皮转染人乳头瘤病毒16型之后的表达变化
Author: Lianqin LI ¹ ; Yonggang ZHANG ; Chunling CHEN ; Zhan WANG ; Shaobing ZHAN
Author Information

1. 滨州医学院烟台附属医院妇产科
Keywords: Peroxidases; Cervix Neoplasms; Papillomavirus,human
From: Chinese Journal of Experimental and Clinical Virology 2015;29(1):38-40
CountryChina
Language:Chinese
Abstract: Objective We previously demonstrated that peroxiredoxin 3 (PRX3) was overexpressed in cervical cancer and the expression of PRX3 was positively associated to that of oncogenes E6/E7 of human papillomaviruses (HPV) type 16.The present study was conducted to investigate the link between HPV oncogenes and PRX3,which would be helpful to understanding the mechanism for cervical carcinogenesis.Methods Cervical epithelial cells were cultured and were transfected with recombinant pcDNA3.1 vector containing HPV16 E6/E7.The changes of PRX3 expression were detected by quantitative real time PCR and Western blotting.Results In the epithelial cells which stably expressed HPV16 E6/E7,the expression of PRX3 was significantly down-regulated at both mRNA and protein levels compared to that in the control cells.Conclusion Integration of high risk HPV DNA into the chromosomes of cervical epitheliums induced the change of the PRX3 expression,which might be involved in the initiation of cervical cancer.