The effect of fish oil on non-alcoholic steatohepatitis induced by high fructose and high fat high cholesterol diet in mouse
10.3760/cma.j.issn.1003-9279.2015.01.005
- VernacularTitle:鱼油对高果糖高脂高胆固醇饮食诱导的小鼠非酒精性脂肪性肝炎的影响
- Author:
Zhenjie ZHUANG
1
;
Jing LIU
;
Yinlan LIU
;
Jian YAN
;
Yan LUO
;
Junping SHI
Author Information
1. 杭州师范大学附属医院转化医学中心
- Keywords:
Fish oils;
Fatey liver;
kupffer cells
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate how fish oil rich diet affected nonalcoholic steatohepatitis (NASH) induced by high fructose high fat high cholesterol diet in mouse.Methods 45 C3H mice aged 6 weeks were divided into 3 groups:normal diet group (control),lard rich diet group as model group (lard),fish oil rich diet (fish oil).Mice were sacrificed at the end of week 4,8,16,5 mice at each time point.Blood and liver were collected to test biochemical parameters,liver index,liver pathology and mRNA expression of inflammation associated gene.Results total cholesterol (TC) and serum ALT,AST increased significantly comparing to control (P ＜ 0.001),fish oil intervention inhibited these increases (P ＜0.05).The expression of monocyte chemotactic protein-1 (MCP-1) was upregulated,most dramatically at week 4,in lard group.While the mRNA level of MCP-1 is lower in fish oil group than lard group.TNF-α and TGF-β gene expression also rise at week 8,this trend diminished in fish oil group.HE staining showed apparent steatosis and inflammation infiltration at week 8 and 16 in lard group,while inflammation infiltration was weaker in fish oil group.The expression of kupffer cell marker gene CD68 elevated in lard group,fish oil diet down regulated this elevation.Conclusion Fish oil was able to protect liver from NASH related injury induced by high fructose high fat high cholesterol diet.
