The expression and significance of autophagy related gene in rats with non-alcoholic fatty liver disease
10.3760/cma.j.issn.1003-9279.2015.01.004
- VernacularTitle:自噬相关基因在大鼠非酒精性脂肪性肝病中的表达及意义
- Author:
Yan LUO
1
;
Yan WANG
;
Zhenjie ZHUANG
;
Yinlan LIU
;
Junping SHI
;
Guoqiang LOU
Author Information
1. 310015,杭州师范大学附属医院
- Keywords:
Fatty liver;
Genes;
Rats
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of autophagy related gene in rats with non-alcoholic fatty liver disease (NAFLD).Methods In vivo model of NAFLD was established in SD rats by high fat diet (model group,n =24),while the rats with normal food were set as control group (n =18).The rats of each group were sacrificed at the end of 4,8,12 weeks respectively.Body weight,liver wet weight,liver function,liver lipid metabolism and other indicators were measured.Hepatic histology was evaluated by hematoxylin-eosin (HE) staining and oil red O staining.The identification of autophagy were morphologically visualized by transmission electron microscopy (TEM).The expression of autophagy related gene ATG7,beclin1,microtubule-associated protein 1 lightchain3 (LC3) were detected by real-time quantitative RT-PCR (qRT-PCR) for mRNA levels.Result ① Compared with the control group at the corresponding time point,the weights of the mice and their livers.②Hepatic oil red O staining areas and density in high fat diet group all increased.HE staining revealed different degrees of macrosteatosis accompanied with intralobular inflammatary foci.With a remarkable histological change of NASH (typical hepatocellular ballooning and perisinusoidal fibrosis) about 80％ of mice from high fat diet group had NASH [NAFLD activity score (NAS) ≥5] at the end of 12 weeks.③ TEM pictures showed that autophagy bubble appeared since high fat diet feeding for 4 weeks.④ The relative expression of ATG7,Beclin1,LC3-Ⅰ,LC3-Ⅱ at mRNA level were up-regulated after high fat diet,and reached their peak at the end of 8 weeks when compared with the control group.The differences can be applied to statistical significance (P ＜ 0.05).The expression of these autophagy related genes in rats of high fat diet group were down-regulated at the end of 12 weeks,which had no difference the control group.Conclusion ①High fat diet feeding is able to induce a rat model of NAFLD.②The expression level of autophagy related genes is altered during high fat diet treatment.③Autophagy may take part in the occurrence and development of NAFLD.
