The detection of Th17 and transcription factors in patients with chronic hepatitis B
10.3760/cma.j.issn.1003-9279.2015.01.002
- VernacularTitle:慢性乙型肝炎患者外周血Th17细胞及相关转录因子表达的检测
- Author:
Simin YAO
1
;
Yingxia LIU
;
Xiaohe LI
;
Jing YUAN
;
Jieyun ZHANG
;
Xiuyun ZHU
;
Mingxia ZHANG
Author Information
1. 深圳市第三人民医院
- Keywords:
Hepatitis B virus;
T-lymphocytes,helper-inducer;
Th1 cells;
Th2 cells;
Interlecking
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(1):2-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the immune response of T helper cells 17 in patients with hepatitis B virus (HBV) and the relation with Th1,Th2 and Treg cells.Methods The percentages of Th17 cells were detected by intracellular cytokine staining with flow cytometry.Using Realtime PCR method,we assayed the mRNA expression of IL-17,T-bet,GATA-3 and FoxP3 in 58 HBV patients and 40 healthy controls in our research.Results Compared with control group (3.56％ ± 1.26％),the percentage of Th17 cells in the peripheral blood of HBV group (4.72％ ± 1.80％) was higher (P ＜0.001).The mRNA of IL-17 expression of the HBV group (4 ±0.49) was higher than in healthy control group (1.19 ±0.19,P ＜ 0.0001).The mRNA of T-bet expression of the HBV group (2.27 ± 0.24) was higher than in healthy control group (1.10 ± 0.13,P ＜ 0.05).The mRNA of GATA-3 expression of the HBV group (2.29 ± 0.16) was higher than in healthy control group (1.10 ± 0.10,P ＜ 0.0001).The mRNA of FoxP3 expression of the HBV group (2.03 ±0.15) was higher than in healthy control group ((1.05 ±0.10,P ＜ 0.0001).Conclusions The Th17 cells participated in immune response of the hepatitis B virus infection patients,and there has certain adjustment relations among Th1,Th2 and Treg cells.
