Effects of Yupingfeng San containing Radix Hedysari or Radix Astragali on the splenic lymphocyte activation in SAMP8 mice
10.3969/j.issn.1006-2157.2017.04.009
- VernacularTitle:含红芪、黄芪玉屏风散对SAMP8小鼠脾淋巴细胞活化影响
- Author:
Meihua SUN
1
;
Wei LI
;
Wenjun ZHANG
;
Qianxiang ZHOU
;
Weidong CHENG
Author Information
1. 南方医科大学 广东 510000
- Keywords:
Yupingfeng powder;
Radix Hedysari;
Radix Astragali;
splenic lymphocyte;
immunosenescence;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(4):308-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of Yupingfeng San (YPFS, Jade Wind-Barrier Powder) containing Radix Hedysari (sweetvetch root, hongqi) or Radix Astragali (astragalus root, huangqi) on the splenic lymphocyte activation in SAMP8 mice.Methods In vitro experiment: Yupingfeng San containing Radix Hedysari or Radix Astragali and saline of the same volume were given to young mice respectively via intragastric administration for 14 days to prepare dosed serum for co-culture with SAMP8 mice spleen lymphocytes;In vivo experiment: Yupingfeng San containing Radix Hedysari or Radix Astragali, thymus peptide solution and saline of the same volume were given to SAMP8 mice respectively for 14 days before their blood and spleen samples were taken for preparing dosed serum and splenic lymphocyte suspension.Levels of TNF-α in vitro and in vivo were measured with ELISA;expression of CD28 mRNA and p38MAPK mRNA in vitro and in vivo were measured by using real-time PCR and the expression of CD28 and p38MAPK by using Western blot.Results Compared with blank serum group and normal saline group, in vitro and in vivo levels of TNF-α were increased in dosed serum groups.So were the expression of splenic lymphocyte CD28, p38MAPK mRNA and protein expression.In vitro levels of TNF-α and CD28, p38MAPK mRNA expression in the hedysari group were higher than those in the astragali group (P<0.01);in vivo levels of TNF-α and CD28, p38MAPK mRNA expression in the astragali group were higher than those in the hedysari group (P<0.05).CD28 and p38MAPK mRNA expression in the hedysari group were higher than that in the astragali group (P<0.05).Changes of TCM groups were inferior to that of the thymosin group (P<0.05).Conclusion Yupingfeng San containing Radix Hedysari or Radix Astragali both played a potential role in the splenic lymphocyte activation of SAMP8 mice, which serve as a certain resistance to immunosenescence.
