Genotypes of interleukin 28B polymorphism and its relationship with responses to therapy in patients with chronic hepatitis C in Guangdong
10.3760/cma.j.issn.1003-9279.2014.06.019
- VernacularTitle:广东地区慢性丙型肝炎患者IL28B基因多态性与抗病毒疗效的关系
- Author:
Fengxia GUO
1
;
Xiayi ZHANG
;
Yun LAN
;
Jianping LI
;
Binbin CHEN
;
Yujuan GUAN
Author Information
1. 510060,广东省广州市第八人民医院肝二科
- Keywords:
Hepatitis C,chronic;
Interleukins;
Polymorphism,single nucleotide;
Interferons alfa2b;
Ribavirin
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(6):458-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotypes of interleukin (IL) 28B polymorphism and the effect of IL28B genotypes on the responses to therapy in patients with chronic hepatitis C (CHC).Methods A total of 74 patients with CHC were prospectively treated with pegylated interferon α (PEG-IFN α) in combination with ribavirin (RBV) for 48 or 72 weeks.After finishing the therapy,the patients were followed up for 24 weeks and the therapeutic effect was evaluated with rapid virological response (RVR) and sustained virological response (SVR).The IL28B rs8099917,rs12979860 and rs12980275 were identified by sequencing the PCR products amplified from the genome DNA of each participate.The IL28 B genotypes in CHC patients were analyzed and the effect of singlenucleotide polymorphism (SNP) on responses to therapy was assessed.Result rs8099917 TT,TG,GG were 63(85.1％),11(14.9％),0(0％),respectively.rs12979860 CC,CT,TT were 60 (81.1％),14 (18.9％),0 (0％),respectively.rs12980275 AA,AG,GG were 57 (77.0％),17 (23.0％),0 (0％),respectively.In patients infected with HCV genotype-1,only rs12979860 was associated with SVR rate,and the proportion of rs12979860 CC in SVR group was significantly higher than that in non-SVR group (88.4％ vs 58.3％,P ＜ 0.05).In patients infected with HCV non genotype-1,none of the three SNPs was associated with RVR or SVR (P ＞ 0.05).Conclusions The majority of the patients with CHC carry IL28B genotype rs8099917 TT,rs12979860 CC and rs12980275 AA in Guangdong.The genotypes of IL28 B(rs12979860) is closely related to the effect of PEG-IFN α/RBV therapy,and it might be an important predictive factor for SVR before treatment in patients with CHC.
