The clinical significance of up-converting phosphor immunochromatography in detecting hepatitis B virus large envelope protein in various types of chronic liver disease
10.3760/cma.j.issn.1003-9279.2014.06.017
- VernacularTitle:上转发光免疫层析法检测乙肝病毒外膜大蛋白在不同类型慢性肝病中的临床意义
- Author:
Miaochan WANG
1
;
Jianchun GUO
;
Qingqing WANG
;
Aifang XU
Author Information
1. 浙江大学免疫研究所
- Keywords:
Viral envelope proteins;
Hepatitis B;
Liver cirrhosis;
Liver neoplasms
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(6):452-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance of up-converting phosphor immunochromatography in detecting hepatitis B virus large envelope protein (HBV-LP) in serum of chronic hepatitis B (CHB),liver cirrhosis (LC) and primary hepatic carcinoma (PHCC) patients.Methods A new UPT-based immunochromatographic technology was employed to detect hepatitis B virus large envelope protein (HBV-LP) in serum of 260 CHB,190 LC and 45 PHCC patients; chemiluminescence was adopted to detect HBeAg; real-time fluorescent quantitative PCR was utilized to detect hepatitis B virus DNA (HBV DNA).Results The majority of CHB patients have high concentrations of HBV-LP (＞40 U/ml) ; most LC and PHCC patients have low concentrations of HBV-LP (＜ 10 U/ml) ; CHB patients,LC patients and PHCC patients are all ranked in descending order in terms of the positive rates of HBeAg,HBV DNA and HBV-LP; the positive rates of HBeAg and HBV DNA in LC and PHCC patients are lower than those in CHB patients and the difference is statistically significant (P ＜ 0.01); within the groups of LC and PHCC patients,the positive rate of HBV-LP is significantly higher than those of HBV DNA and HBeAg and the difference is statistically significant (P ＜ 0.05).Conclusion As a swift and reliable method in detecting HBV-LP levels,up-converting phosphor immunochromatography is of great value in diagnosing chronic liver disease and monitoring treatment effects.
