Regulation effect of Kudiezi Injection on PKCδ/MARCKS signal pathway in vascular endothelial cells after hypoxia injury
10.3969/j.issn.1006-2157.2017.03.007
- VernacularTitle:苦碟子注射液对缺氧损伤血管内皮细胞PKCδ/MARCKS通路的调节
- Author:
Jiayi YANG
1
;
Jialan YU
;
Zhong DAI
;
Hongsheng LIU
;
Wen BAI
Author Information
1. 北京大学人民医院 北京 100044
- Keywords:
Kudiezi Injection;
hypoxia injury;
vascular endothelial cells;
PKCδ/MARCKS
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(3):214-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of Kudiezi Injection (KI) on vascular endothelial cells, and study initially the effective mechanism of PKCδ/MARCKS signal pathway after hypoxia injury.Methods The hypoxia injury model was induced by CoCl2 in human umbilical vein endothelial cells (HUVEC), and then divided into normal group, model group, KI group and Rottlerin group.The cytomorphologic changes were observed by using light microscope 24 h after hypoxia injury, vitality of HUVEC was detected by using methyl thiazolyl tetrazolium test (MTT), and distributions and expressions of p-PKCδ and p-MARCKS were detected by using immunocytochemical method and Western Blot in all groups.Results KI and Rottlerin alleviated significantly the cytomorphologic changes, and enhanced significantly the vitality of cells after hypoxia injury (P<0.01).The expressions of p-PKCδ and p-MARCKS were significantly increased in model group compared with normal group (P<0.05), and were significantly inhibited by KI and Rottlerin (P<0.05).Conclusion KI has a protective effect on vascular endothelial cells after hypoxia injury, and the mechanism is related to down-regulation of vitality of PKCδ/MARCKS signal pathway.
