Study on chemical constituents in Cordyceps hawkesii by UPLC-Q-TOF-MS
10.3969/j.issn.1006-2157.2017.02.014
- VernacularTitle:基于UPLC-Q-TOF-MS法分析亚香棒虫草化学成分
- Author:
Weihan QIN
1
;
Fei LIU
;
Xiang LIU
;
Xiaomei ZHANG
;
Yong YANG
Author Information
1. 重庆市中药研究院 重庆 400065
- Keywords:
Cordyceps hawkes;
ultra performance liquid chromatography-qudropole time-of-flight mass spectrometry;
cleavage law;
qualitative analysis
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(2):159-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the main chemical constituents of extract from Cordyceps hawkesii by using method of ultra performance liquid chromatography-qudropole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Methods UPLC condition was as the following: Agilent SB-C18 (50 mm×4.6 mm, 1.8 μm) column with gradient elution consisting of 0.1 % formic acid in water and acetonitrile, at a flow rate of 0.25 mL/min.The column temperature was set at 30℃.MS analysis was based on information associated mode (IDA) that positive and negative ions were respectively collected.Results Mass spectrometry combined with "Formula Finder" of PeakView software and database, a total 26 compounds were identified as alkaloids, glycosides, nucleosides, and amino acids.9 of them had never been reported in Cordyceps hawkesii, and 2 of them were known.Conclusion The method is accurate, reliable and efficient for rapid identification of compounds in Cordyceps hawkesii.The results provide references for the development and utilization of Cordyceps hawkesii, as well as finding out its pharmacodynamic material basis.
