Dynamic changes of chemical compositions in volatile oil extracted from Menthae Haplocalycis Herba during different harvest periods using HS/SPME-GC-MS
10.3969/j.issn.1006-2157.2017.02.013
- VernacularTitle:HS/SPME-GC-MS法分析不同采收期薄荷药材挥发油成分动态变化
- Author:
Qiaolu LYU
1
;
Wei WU
;
Sensen CHI
;
Bin LIU
Author Information
1. 北京中医药大学中药学院 北京 100102
- Keywords:
Menthae Haplocalycis Herba;
headspace solid phase micro-extraction;
GC-MS;
volatile oil;
harvest period
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(2):155-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the chemical compositions of volatile oil extracted from Menthae Haplocalycis Herba (mint) collected during different harvest periods by using headspace solid phase micro-extraction coupled with gas chromatography-mass spectrometry (HS/SPME-GC-MS).Methods The volatile oils were extracted by the method of headspace solid phase micro-extraction, then the chemical compositions were determined by using GC-MS and quantified by the area normalization method.Results A total of 47 compositions were detected in Menthae Haplocalycis Herba in mature stage, and 12 of those had been identified with the total content to be 78.23%.By comparing with the relative amount of menthone, l-limonene and l-menthol extracted from the herbal medicine samples collected during different harvest periods, which are the key components in biosynthesis pathway of the herb, the preliminary determination of the best time to harvest was September.Conclusion The effective and rapid method for the determination of volatile oils provided basis for studying the dynamic changes of the chemical compositions of volatile oil extracted from Menthae Haplocalycis Herba and the proper time to harvest.
