Effect of A(c)aí on cyclic nucleotides, neurotransmitters and immune level of rats with asthenia pyrosyndrome
10.3969/j.issn.1006-2157.2017.02.012
- VernacularTitle:阿萨伊对虚热证大鼠环核苷酸、神经及免疫水平的影响
- Author:
Zichen WANG
1
;
Linyuan WANG
;
Yingli ZHU
;
Xiaoran TANG
;
Sha WANG
;
Yan QU
;
Jianjun ZHANG
Author Information
1. 北京中医药大学基础医学院 北京 100029
- Keywords:
A(c)aí;
asthenia pyrosyndrome;
property of Chinese herbal medicine;
cyclic nucleotides;
neurotransmitter;
immunities;
complement;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(2):148-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of frozen fruit pulp of A(c)aí (Euterpe Oleraceae Mart.) on cyclic nucleotides, neurotransmitters and immune level of rats with asthenia pyrosyndrome.Methods SD rats were randomly divided into blank group, model group, Huangbo (Cortex Phellodendri Chinensis) group, Gegen Qinlian Decoction (a Chinese herbal medicine compound) group, A(c)aí high-dose group and A(c)aí low-dose group.The rat model of asthenia pyrosyndrome was induced by intramuscular injection of dexamethasone sodium phosphate at dose of 0.35 mg/kg once daily for consecutive 21 days.The level of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were detected by radioimmunoassay.The levels of dopamine (DA), dopamine beta-hydroxylase (D-β-H) and norepinephrine (NE) were detected by enzyme immunoassay.The levels of IgG, IgM, C3 and C4 were detected by using colorimeter.The results of the groups were compared to analyze the effect of Huangbo, Gegen Qinlian Decoction and A(c)aí on rats with asthenia pyrosyndrome.Results High dose A(c)aí could relieve the symptoms due to asthenia pyrosyndrome, and decreased the serum level of cAMP, cGMP, cAMP/cGMP, DA, D-β-H and NE (P<0.05), while increased the serum content of IgG, IgM and C3 (P<0.05,P<0.001 or P<0.05).Conclusion The effect of A(c)aí on cyclic nucleotides, neurotransmitters and immune level was similar with that of Huangbo or Gegen Qinlian Decotion, which proved the cold property of A(c)aí.
