Preliminary study of lung function affected by spleen deficiency pattern combined with pulmonary fibrosis of rats
10.3969/j.issn.1006-2157.2017.02.009
- VernacularTitle:脾虚复合肺纤维化影响大鼠肺功能的初步研究
- Author:
Man ZHU
1
;
Di KANG
;
Hui ZHANG
;
Yu LI
;
Lei TANG
;
Mengmeng SHEN
;
Chunfang HUANG
;
Jifeng WANG
;
Jianzhao NIU
;
Yadong LI
Author Information
1. 北京中医药大学基础医学院 北京 100029
- Keywords:
bleomycin;
rheum officinale;
restriction of ventilation function;
organ index;
FVC/lung weight;
FEV0.4/FVC％;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(2):130-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of spleen deficiency syndrome induced by rhubard solution combined with lung fibrosis induced by bleomycin on lung function of rats, so as to discuss the relationship between spleen deficiency syndrome (SDS) and pulmonary fibrosis.Methods SD rats were randomly divided into normal group, SDS group, model group, SDS + lung fibrosis group.The rat SDS model was induced by intragastrical administration of rheum officinale with fasting every other day for 30 days.Then the rats were infused bleomycin(5 mg/kg)through tracheal intubation to simulate the lung fibrosis disease with SDS.Four weeks after injection of bleomycin, rats' lung function was detected, lung index and spleen index were calculated, and the pathological changes of lung tissue and aiway were observed by using HE staining and Masson staining.Results Compared with the normal group, weight of lung, lung index, FEV0.4/FVC% were increased in the model group(P<0.05), and the lung dynamic compliance (Cdyn), forced vital capacity (FVC), FVC /lung weight decreased, peak expiratory flow rate (PEF) as well as maximal mid-expiratory flow rate (MMF) decreased in model group(P<0.05).What bleomycin caused was characterized by airway centered interstitial fibrosis.In the small airway, the shed epithelial cells and mucus plugs could been seen.The lung function of rats of SDS group had no difference with that of the healthy cats.Compared with the model group, FEV0.4/FVC% significantly increased (P<0.05) and the area ratio of interstitial fibrosis increased in the SDS+ lung fibrosis group.Conclusion Spleen deficiency syndrome is a potential adverse factor to aggravate restriction of ventilation function due to bleomycin.
