Effect of Xiaoyin Jiedu Fangon JAK1/STAT3 signaling pathway in Jurkat T cells
10.3969/j.issn.1006-2157.2017.02.007
- VernacularTitle:消银解毒方颗粒对Jurkat T细胞内JAK1/STAT3信号通路作用机制的研究
- Author:
Xi CHEN
1
;
Chunbo HUO
;
Guangshan CHEN
;
Yunbi ZHANG
;
Xingwu DUAN
;
Runtian ZHANG
;
Lingling LI
Author Information
1. 北京中医药大学东直门医院东区 北京 101100
- Keywords:
Xiaoyin Jiedu Fang guanule;
Jurkat T cells;
JAK1/STAT3 signaling pathway;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(2):119-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Xiaoyin Jiedu Fang, a Chinese medicine compound formulated by TCM principle of cooling blood with detoxification, on nonreceptor tyrosine kinase JAK1/ signal transducer and activator of transcription 3 (STAT3) signaling pathway in human acute leukemia Jurkat T cells.Methods The abnormal activation of T cells of psoriasis with TCM pattern of blood-heat was induced by stimulating Jurkat T cells with phytohaemagglutinin (PHA) and IL-6.The medicated serum contained Xiaoyin Jiedu Fang granule (XYJDF), Liangxue Fang (LXF) or Jiedu Fang granule (JDF) which was a disassembled prescription of XYJDF, acitretin (positive drug) and distilled water (negative control) were prepared.The model cells were inoculated into 24-hole cell culture plate and were cultured with the prepared medicated serum and Filgotinb(GLPG0634), an inhibitor of JAK/STAT signaling pathway for 48 hours.Jurkat T cells with no treatment was served as the blank group.Protein and mRNA expression of JAK1, STAT3, glucoprotein(GP)130 were detected by using Western blot and real-time PCR.Results The protein and mRNA expression of JAK1,STAT3 and GP130 in the model group were significantly higher than those of the blank group (P<0.01).Compared with the model group, the protein expression of pJAK1/JAK1, pSTAT3/STAT3 and GP130 in all medicated group were decreased significantly (P<0.01), and the mRNA expression of JAK1, STAT3 and GP130 declined (P<0.01 or P<0.05).Conclusion The effect of XYJDF against psoriasis could be via the way of inhibiting JAK1/STAT3 signaling pathway leading to prevent abnormal activation of T cells.
