Genotyping and mutational analysis of occult hepatitis B virus infection in blood Donors in Quzhou

VernacularTitle:衢州市无偿献血者中隐匿性HBV感染的基因型别及突变类型分析
Author: Hao LV ¹ ; Sujun JIANG ; Aihua SUN
Author Information

1. 浙江省衢州市中心血站
Keywords: Hepatitis B virus; Blood donors; Infection; Genotype
From: Chinese Journal of Experimental and Clinical Virology 2014;28(5):371-373
CountryChina
Language:Chinese
Abstract: Objective To assess the molecular biological characteristics of occult hepatitis B virus (HBV) infection in blood donors in Quzhou.Methods A total of 24 178 blood samples from the free-of-charge blood donors were enrolled in the study,all of donations were tested by two different HBsAg ELISA.The occult HBV infection (OBI) was determined by DNA analysis among the HBsAg negative samples.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 24 020 HBsAg negative samples out of 24 178 blood donors,15 were HBV DNA positive.The occult HBV infection rate was 0.62‰ (15/24 020).Among the 15 samples,9 were genotype C (60％) and 6 genotype B (40％).In addition,a specific mutation in "a" epitope was observed in 11 OBI virus strains by amino acid analysis.Conclusion There was occult HBV infection among blood donors in Quzhou,which is probably associated with the gene mutation of HBV virus.