Effect of Qili Qiangxin Capsule on myocardial fibrosis and TGF-β1/Smad3 signaling pathway in rats with myocardial infarction
10.3969/j.issn.1006-2157.2017.01.009
- VernacularTitle:芪苈强心胶囊对心肌梗死大鼠心肌纤维化及TGF-β1／Smad3信号通路的影响
- Author:
Anbang HAN
1
,
2
;
Jian ZHANG
;
Yingdong LU
;
Mingjing ZHAO
;
Yizhou ZHAO
;
Yaoyao HE
;
Xiangning CUI
Author Information
1. 中国中医科学院广安门医院 北京 100053
2. 北京中医药大学
- Keywords:
acute myocardial infarction;
myocardial fibrosis;
Qili Qiangxin Capsule;
TGF-β1 /Smad3 signaling pathway;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(1):41-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of Qili Qiangxin Capsule (QQC)on myocar dial fibrosis of rats with myocardial infarction(MI).Methods Acute myocardial infarction (AMI)mod-el was induced by ligation of the proximal left anterior descending branch of coronary artery of SD male rats for four weeks.Then the survival rats with AMI were randomly divided into model group (n =12)or QQC group (n =9)according to the transthoracic echocardiography results,and 10 more rats were as-signed into sham operation group.Rats of QQF group were intragastrically administered QQC at the dose of 1 g/kg once daily,while those of the other two groups took the same volume of distilled water,for con-secutive four weeks.The cardiac function was measured using echocardiography,the myocardial tissue close to infarction was observed with HE or Masson staining,α-SMA expression in myocardial tissue was detected using immunohistochemistrical method,and protein expression of α-SMA,collagen I,transfor-ming growth factor-β1 (TGF-β1 )and p-Smad3 were detected using Western blotting.Results Compared with the sham operation group,left ventricular fractional shortening (FS)and ejection fraction (EF)of model group were significantly lower (P <0.05),left ventricular end systolic diameter (LVID)and end systolic volume (ESV)increased significantly (P <0.05 ),myocardial fibrosis ratio was significantly higher (P <0.05),and the protein expression of α-SMA,collagenⅠ,TGF-β1 and p-Smad3 were higher (P <0.05 ).Compared with model group,EF and FS of QQF group were significantly lower (P <0.05),LVID and ESV were significantly higher(P <0.05),the degree of myocardial fibrosis was re-lieved,and α-SMA,collagenⅠ,TGF-β1 ,p-Smad3 protein expressions in myocardium were lower(P <0.05).Conclusion Qili Qiangxin Capsule was demonstrated to improve the cardiac function of rats with AMI by inhibiting the process of fibrosis remodeling,and the effects may be mediated through regu-lating TGF-β1 /Smad3 signaling pathway.
