Influence of electro-acupuncture of different frequency and stimulation times on skin blood perfusion around Neiguan (PC6)and sham acupoint in rats with myocardial ischemia
10.3969/j.issn.1006-2157.2016.12.001
- VernacularTitle:不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响
- Author:
Yawen LU
1
;
Bo JI
;
Jian DAI
;
Guozhen ZHAO
;
Dan WANG
;
Mingna YAN
;
Hongxin BAI
;
Xi-Aomin SUN
;
Mengwei GUO
;
Yingqiu MAO
;
Zhigang LI
;
Kai CHENG
Author Information
1. 北京中医药大学针灸推拿学院 北京 100029
- Keywords:
myocardial ischemia;
electro-acupuncture;
Neiguan(PC 6);
sham acupoint;
skin blood per-fusion;
correlated acupoints and viscera;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(12):1041-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the skin blood perfusion around Neiguan (PC 6)and sham acupoint needled by the different frequency and stimulation times of electro-acupuncture (EA),in healthy rats and myocardial ischemic rats.Methods According to random number table,altogether 100 male Wistar rats were divided into once treatment group and three times treatment group,which were both divided into control group,sham-operation group,model group,EA low-frequency group and EA high-frequency group (each n =10).After successful modeling,rats of EA groups were acupunctured at left Neiguan (PC 6)acupoint.60 minutes after last treatment of acupuncture,skin blood perfusion around bilateral Neiguan(PC 6)acapoints and sham acupoint were tested by laser speckle contrast imaging technology (LSCI) and analyzed,and the myocardial morphological changes were observed by HE staining. Results Compared with control group,the skin blood perfusion around PC 6 in model groups decreased significantly after three time treatment (P <0.01 ),and that around PC 6 or sham acupoint in model group of three times treatment group was much less than that of once treatment group (P <0.01).Com-pared with the model group,the skin blood perfusion around PC 6 both significantly increased after low-frequency EA or high-frequency EA after three times treatment (P <0.01).Conclusion The variation of skin blood perfusion around PC 6 should be useful for specific reflection of normal condition or ische-mic condition of rats myocardium,but that around sham acupoint didn’t.The effect of three-times-treat-ment against declination of skin blood perfusion due to myocardial ischemia was superior to that of once-treatment regardless of high frequency or low frequency,and EA of high frequency and three-times stimu-lation showed the significant effect.However,the mechanism remains to be further studied.
