Effect of wind-dispelling drugs and deficiency-tonifying drugs on axonal growth inhibitory signaling pathway Nogo-A/NgR and RhoA/Rock2 after cerebral ischemia in rats:a developmental study on Hou’s Hei San
10.3969/j.issn.1006-2157.2016.12.009
- VernacularTitle:侯氏黑散中风药、补虚药对脑缺血大鼠轴突生长抑制信号通路Nogo-A／NgR与RhoA／Rock2的影响
- Author:
Yue LU
1
;
Hui ZHAO
;
Xiaoquan YAO
;
Jiahui CHANG
;
Qiuxia ZHANG
Author Information
1. 首都医科大学中医药学院 北京 100069
- Keywords:
cerebral ischemia;
wind-dispelling drugs;
deficiency-tonifying drugs;
Nogo-A /RhoA /Rock2 signaling pathway;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(12):1017-1021
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San,a TCMformula used in treatment of apoplexy,on axonal growth inhibitory factors Nogo-A,NgR,RhoA,Rock2 of rats with cerebral ischemia.Methods The model of permanent middle cere-bral artery occlusion (pMCAO)was induced by using Longa method.All rats were randomly divided in-to sham-operation group,model group,wind-dispelling drugs group(7.70 g/kg),deficiency-tonifying drugs group(2.59 g/kg)and combined drugs group(10.50 g/kg).After operation,each 12 h the rats were intragastrically administered for 72 h.Then the rats were sacrificed and their brain tissue were re-sected.The protein expression of Nogo-A,NgR,RhoA and Rock2 in hippocampus CA1 were detected by using Western blot.Results The protein expressions of Nogo-A,NgR,RhoA and Rock2 in model group increased significantly,compared with sham-operation group(P <0.01 ).Compared with model group, the expression of NgR in wind-dispelling drugs group,deficiency-tonifying drugs group and combined drugs group decreased remarkably(P <0.01).The expression of Nogo-A and Rock2 decreased as well, but only combined drugs group showed a statistically difference(P <0.05).Conclusion The effect of wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San against cerebral ischemia damage would be via regulating axonal growth inhibitory signal pathway Nogo-A /RhoA /Rock2 to promote the re-covery of neural function.
