Effect of Qingjin Huatan Decoction on Foxp3 and RORγt in lung tissue of rats with acute exacerbation of chronic obstructive pulmonary disease
10.3969/j.issn.1006-2157.2016.12.007
- VernacularTitle:清金化痰汤对慢性阻塞性肺疾病急性加重期模型大鼠肺组织 Foxp3和 RORγt 表达的影响
- Author:
Jianchao DU
1
;
Cuiling FENG
;
Dongyu GE
;
Qian ZHANG
;
Dongmei CHEN
;
Xiaoya ZHANG
;
Bei JIA
Author Information
1. 北京中医药大学东直门医院 北京 100700
- Keywords:
acute excerbation of chronic obstructive pulmonary disease;
airway mucus hypersecretion;
Qingjin Huatan Decoction;
forkhead box P3;
retinoid-related orphan nuclear receptor γt;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(12):1006-1012
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Qingjin Huatan Decoction on forkhead box P3 (Foxp3) and retinoid-related orphan nuclear receptor γt (RORγt)in the lung tissue of airway mucus hypersecre-tion rat model with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods Altogether 40 male Wistar rats,SPF grade,were randomly divided into normal group,model group, Qingjin Huatan Decoction group and clarithromycin group.AECOPD rat model was induced by intratra-cheal instillation of lipopolysaccharide (LPS)with passive smoking.From the first day of the experiment, rats of model group were intragastrically administered saline,and those of Qingjin Huatan Decoction group and clarithromycin group took corresponding medicines at dose of 8.4 g/kg and dose of 50 mg/kg for consecutive 30 days.On day 31,all rats were sacrificed.The thin sections of the rats’resected lungs were made,the expressions of mucin 5AC (Muc5AC),neutrophil elastase (NE),Foxp3 and RORγt in lung tissue were detected by using immunohistochemical method,and the protein expressions of Foxp3 and RORγt were measured by using Western blot.Results Compared with the normal group,Foxp3 protein expression in model group had no significant difference (P >0.05),but expression of RORγt, Muc5AC and NE increased significantly with Foxp3 /RORγt decreased significantly (P <0.01 ).Com-pared with model group,Foxp3 expression in Qingjin Huatan Decoction group had no significant differ-ence (P >0.05),but expression of RORγt,Muc5AC and NE decreased significantly with Foxp3 /RORγt increased significantly (P <0.01 or P <0.05 ).Compared with clarithromycin group,Muc5AC de-creased significantly in Qingjin Huatan Decoction group (P <0.05).Conclusion The potential mecha-nism of Qingjin Huatan Decoction against AECOPD mucus hypersecretion was to downregulate RORγt protein expression in lung tissue and to upregulate Foxp3 /RORγt.
