Mechanism of tetramethylpyrazine in treatment of coronary heart disease based on the coexpression-protein interaction network
10.3969/j.issn.1006-2157.2016.12.005
- VernacularTitle:基于共表达蛋白相互作用网络探讨川芎嗪治疗冠心病的机制
- Author:
Mengqi HUO
1
;
Yanling ZHANG
;
Shichao ZHENG
;
Yanjiang QIAO
Author Information
1. 北京中医药大学中药信息工程研究中心 北京 100102
- Keywords:
tetramethylpyrazine;
coronary heart disease (CHD);
function modules;
mechanism
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(12):989-997
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of tetramethylpyrazine in the treatment of coronary heart disease (CHD)based on the coexpression-protein interaction network analysis.Methods The tar-gets’information of tetramethylpyrazine was obtained from pharmacophore-based virtual screening and da-tabase searching from STITCH &Chemprot,and the protein-protein interactions of the targets were re-trieved by String database.The protein interaction network was constructed by Cytoscape.Next,the co-expression-protein interaction networks were constructed by integrating gene-expression profile under physiological and disease conditions.The modules of disease associated coexpression-protein interaction network were clustered and functional annotated based on fast agglomerate algorithm based on the edge clustering coefficients (FAG-EC)and gene ontology (GO)enrichment,by which the function modules were mapped to the coexpressin-protein interaction network under the normal physiological state to get the corresponding function modules.Then the function modules under two different conditions were compared and analyzed.Results The effect of tetramethylpyrazine on CHD was achieved by means of regulating and controlling immunological regulation or inflammatory response,cell apoptosis signaling pathway, blood circulation,heme metabolism and basal metabolism;myeloperoxidase (MPO)and hemeoxygenase-1 (HMOX1)probably were two key targets in treatment of CHD.Conclusion The mechanism and the key targets of tetramethylpyrazine in the treatment of CHD were illuminated at the molecular networks lev-el,which could provide reference for clinical practice.
