Dengue virus-derived piRNAs in Aedes albopictus
10.3760/cma.j.issn.1003-9279.2014.04.012
- VernacularTitle:白纹伊蚊感染登革病毒后病毒特异性piRNAs分析
- Author:
Zhifa LAI
1
;
Yanhai WANG
;
Jinbao GU
;
Xiaoguang CHEN
Author Information
1. 518040,深圳市福田区疾病预防控制中心
- Keywords:
Dengue virus;
Aedes;
RNA
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(4):277-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and analyze Dengue virus-derived vsRNAs and piRNAs in Aedes albopictus.Methods Female adults Aedes albopictus were collected 2-4 days post-emergence and were injected with Dengue type 2 virus NGC strain,while control group were injected with equal volume of physiological saline.Total RNA was isolated,and small RNAs ranging up to 30 nt in length were excised and analyzed using Illumina HiSeq 2000.After removing adaptor sequences and contaminated reads,Clean reads were aligned to Dengue virus genome and its complementary sequence by SOAP2.The length,strand ratio nucleotide bias and genome distribution of Dengue virus-derived vsRNAs and vpiRNAs were further analyzed.Results Total,3835 vsRNAs and 395 vpiRNAs unique tags were obtained,among them 94.99％ vpiRNAs were derived from virus genome strand,and distributed across the viral genome,but with an enrichment at several "hot-spots".Except weak bias for adenine at position 10 (10A)in the sense molecules as the feature of secondary piRNA,other signature piRNA characteristics were not observed,including a preference for a uridine at their 5'-end,which were main characteristics of primary piRNAs,and a significant 10 nt overlap (Ping-Pong)between sense and antisense piRNA.Conclusion Dengue virusderived piRNAs were indentified in Dengue virus infected Ae.albopictus.
