Myxoma virus induces apoptosis via PI3K/AKT pathway in endometrial cancer cells
10.3760/cma.j.issn.1003-9279.2014.04.010
- VernacularTitle:黏液瘤病毒作用PI3K/AKT通路诱导子宫内膜癌细胞凋亡
- Author:
Yun ZHOU
1
;
Keke XU
;
Rong GUO
;
Min HUANG
;
Hongjie SUN
Author Information
1. 518010,深圳市第五人民医院妇产科
- Keywords:
Endometrial neoplasms;
Apoptosis;
Myxoma virus;
in vitro
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(4):271-273
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis-inducing effect of myxoma virus in the human endometrial cancer HEC-IB cell line.Method A morphological analysis,nuclear condensation,and fragmentation of chromatin were monitored using immunofluorescence staining.Cell viability was assessed using MTT assay.Cell apoptosis and the apoptosis-related activation in the HEC-IB cell line were evaluated by flow cytometry and Western blotting,Result Suppressed the proliferation of the HEC-IB cell line in a time-dependent manner.The obvious inhibiting effect of MV on HEC-IB cells viability and its apoptosisinducing effect were observed.MV treatment downregulated the activation of protein kinase B (AKT) and the expression of glycogen synthase kinase 3 (GSK3),Conclusion MV induced apoptosis involved several molecular pathways.It may suppress constitutively activated targets of phoaphatidylinositol-3-kinases (PI3K) and AKT in the HEC-IB cell line,inhibiting the proliferation and induction of apoptosis.
