Absorbed constituents in rat serum after oral administration of water ex-tract of Simiao Yong’an Decoction
10.3969/j.issn.1006-2157.2016.11.009
- VernacularTitle:四妙勇安汤水提物大鼠吸收入血成分研究
- Author:
Sensen CHI
1
;
Weihua WANG
;
Bin LIU
Author Information
1. 北京中医药大学中药学院 北京 100102
- Keywords:
Simiao Yong’an Decoction;
constituents absorbed in serum;
liquid chromatograph-mass spectrometry-ion trap-time of flight;
metabolites
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(11):933-940
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the absorbed constituents of water extract of Simiao Yong’an Decoction (SYD)in serum of rats,so as to find out the pharmacodynamics material basis.Methods Using liquid chromatograph-mass spectrometry (LCMS)-ion trap (IT)-time of flight (TOF)method under both positive and negative ion modes of electrospray ionization,the water extract of SYD,rats’serum after oral adminis-tration of SYD and the blank serum were analyzed.Serum samples were pretreated by using methanol.The chromatographic condition consisted of Inertsil ODS-3 column,mobile phase of acetonitrile (A)and water (0.1% formic acid contained)(B)with gradient elution.The compounds absorbed in serum was identi-fied by multiple stage mass spectrum fragments,published reports and standard substances.Results Fi-nally,14 compounds were preliminary identified in rats serum,of which ten compounds were prototypes of SYD and four were metabolites.Nine peaks were validated as harpagide,chlorogenic acid,loganin,swero-side,liquiritin,hyperoside,angoroside C,isochlorogenic acid C and harpagoside.Conclusion Constitu-ents of SYD in serum were generated from compatibility of all herbal medicines.This study would contrib-ute to the further study of pharmacodynamics properties and metabolism of SYD.
