Immune regulatory effect of bone marrow mesenchymal stem cells of patient with hepatitis B virus related decompensated cirrhosis
10.3760/cma.j.issn.1003-9279.2014.04.007
- VernacularTitle:失代偿期乙肝肝硬化患者的骨髓间充质干细胞的免疫调节作用
- Author:
Chunhui GUO
1
;
Lanxiu HAN
;
Meirong WAN
;
Jianhe GAN
;
Guojiong DENG
Author Information
1. 东南大学附属江阴医院感染科
- Keywords:
Hepatitis B;
Liver cirrhosis;
Bone marrow cells;
T-Flymphocyte
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(4):262-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of bone marrow mesenchymal stem cells (BMSCs) on autologous peripheral blood lymphocyte proliferation and regulatory T cells (Tregs)subsets in vitro in patients with hepatitis B virus related decompensated cirrhosis.Methods MSCs were isolated and expanded from human bone marrow blood of eight patients with decompensated cirrhosis.The purity of MSCs was identified by flow cytometry.lymphocytes were isolated from the peripheral blood of patients and stained with carboxy fluoresce indiacetate succinimidyl ester (CFDA SE).The BMSCs and peripheral blood lymphocytes from patients were added into wells containing autologous serum in the presence of phytohemagglutinin (PHA).lymphocytes proliferation rate and CD4 + CD25 + CD127-Tregs frequency were detected by flow cytometry.Results Flowcytometric analysis showed that lymphocyte proliferation in contact co-culture group and noncontact co-culture group were much lower than positive control (all P ＜ 0.01),and significantly higher than negative control (all P ＜ 0.01),while CD4+ CD25+ CD127-Tregs in contact co-culture group and noncontact co-culture group were much higher than positive control and negative control (all P ＜ 0.01).Besides,there was no significant difference on lymphocyte proliferation rate or Tregs frequency between contact co-culture group and non-contact co-culture group(all P ＞ 0.05).Conclusion BMSCs can inhibit proliferation of autologous peripheral blood lymphocytes and increase expression of CD4 + CD25 + CD127-Tregsin patients with decompensated cirrhosis.
