Evolutionary relationships of group A rotavirus type G9 isolated from human and pigs in Lulong,Hebei province
10.3760/cma.j.issn.1003-9279.2014.04.004
- VernacularTitle:河北省卢龙地区猪与人G9型A组轮状病毒进化关系研究
- Author:
Xin MA
1
;
Dandi LI
;
Yanqing GUO
;
Jingyao XIANG
;
Xiuping LI
;
Zhaojun DUAN
Author Information
1. 宁夏医科大学
- Keywords:
Rotavirus;
Reassortant viruses
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(4):252-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the main genes molecular characteristics and evolutionary relationship of porcine and human group A rotavirus (GARV) type G9 in Hebei Lulong region.Methods A stool specimen from diarrhea piglet marked as LLP48 in 2008 in Hebei Lulong region,which was G9 GARV positive and 4 stool specimens collected from hospitalized children with diarrhea under the age of five from 2009 to 2011 in Lulong region which were type G9 GARV positive were selected and the main genes were amplified by RT-PCR.After sequencing,the obtained genes were analyzed through MEGA,DNAStar software and other biological sequence alignment,homology and phylogenetic analyses were performed.Results Porcine GARV LLP48 in Lulong region and four strains of Lulong human GARV were highly homologous in VP7,VP6,NSP4 and NSP2 gene fragments,and nucleotide and amino acid homology respectively was 89.4％-94％ and 94.8％-98.2％ ; VP4 fragment of the nucleotide homology (amino acid) is low,71.4％-71.6％ (68.2％-69.0％).Phylogenetic analysis showed that LLP48 genes encoding VP7,VP6,NSP4 and NSP2 were closely related to the strain derived from human source,and VP4 gene closely related to the strains derived from porcine source.Conclusion Lulong porcine GARV LLP48 strain may be a natural reassorted product of porcine VP4 and human VP7,VP6,NSP4 and NSP2.
