Study of liver fibrosis rat model with pattern of liver-qi stagnation and validation of pattern time window by TCM formulas treatment
10.3969/j.issn.1006-2157.2016.11.003
- VernacularTitle:肝纤维化肝气郁结证大鼠模型研究与证候时间窗方剂验证
- Author:
Jinglu YAN
1
;
Xiaoxing XUE
;
Junling LI
;
Kai XIA
;
Tian WANG
;
Wei WANG
Author Information
1. 北京中医药大学 北京 100029
- Keywords:
liver fibrosis;
pattern of liver-qi stagnation;
rat model;
time window of pattern
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(11):893-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the pathogenesis and TCMpatterns related to liver fibrosis ,as well as to establish the rat model of liver fibrosis of combination of disease and pattern.Methods The rat liver fibrosis model with pattern of liver-qi stagnation was induced by subcutaneous injection of carbon tetrachloride.The patterns were distinguished by body weight,open field test,grip strength test,and form collection of symptoms and signs,and the pathological stage was confirmed by HE and Masson staining.Then Chaihu Shugan Powder,Xiaoyao Powder and Sini Powder were used to direct at TCMpat-terns,and colchicine was used to direct at the disease.Results Four weeks after modeling,pattern of liver-qi stagnation occured on the majority of liver fibrosis rats and lasted to the twelfth week.On the oth-er hand,the main pathological stage was S2 to S3 at the fourth week after modeling,S3 at the eighth week and S4 at the twelfth week.The treatment of TCMformulas validated the improvement of symptoms and pathological stages.Conclusion The time window of TCM pattern of liver fibrosis rat model with pattern of liver-qi stagnation was from 4 to 10 weeks,which was proved by TCMformulas.And the model of combination of pattern and disease was reliable.
