Screening of vaccine candidates for enterovirus 71 associated hand, foot and mouth disease
10.3760/cma.j.issn.1003-9279.2014.04.002
- VernacularTitle:肠道病毒71型手足口病的疫苗筛选和观察
- Author:
Simin YAO
1
;
Guilin YANG
;
Weilong LIU
;
Mutong FANG
;
Boping ZHOU
Author Information
1. 518112,深圳市第三人民医院
- Keywords:
Enterovirus;
Hand,foot and mouth disease
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(4):245-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Screen for safe and effective vaccine candidates for EV71,provide a theoretical basis for development of EV71 vaccines in the future.Methods VP1 gene of enterovirus was used to design a target for development of EV71 vaccines.Different vaccine candidates,including inactivated EV71 vaccines,VP1 protein vaccine,DNA vaccines of different doses,were used to challenge female BALB/c mice by intramuscular injection at baseline (0),2 weeks,4 weeks,and caudal vein blood was collected at 0,2,4,6,8,10,and 16 weeks,and BALB/c mice were sacrificed and the spleen cells were collected for detection of both humoral immunity and cellular immunity to evaluate the efficacy and safety of the vaccine candidates.Results IgG antibody titers were increased at 2 weeks,remarkably increased at 4 weeks,reached a peak at 8 weeks,at least sustained for 16 weeks during the whole observation period,subtypes of IgG1 and IgG2a were the major component.The three vaccines could induce cellular immunity characterized by EV71 specific γ-IFN and IL-4 production.Our results indicated that inactivated EV71 vaccine was superior to the other vaccine candidates.Conclusions Inactivated EV71 vaccines,VP1 protein vaccine,DNA vaccines can induce both strong and sustainable humoral and cellular immunities in challenged mice,and the inactivated EV71 vaccine is superior to the other vaccine candidates,which needs to be proved their immunity by challenge assay in the future.
