Effect of electro-acupuncture on Aβin cortex and serum of APP/PS1 transgenic mice
10.3969/j.issn.1006-2157.2016.10.018
- VernacularTitle:电针对 APP／PS1转基因小鼠皮层和血清β淀粉样蛋白的影响
- Author:
Xin WANG
1
;
Zhigang LI
;
Jin CAO
;
Menghan LU
;
Wei LU
;
Quankai JING
;
Yang GAO
;
Weiguo XUE
Author Information
1. 北京中医药大学 北京 100029
- Keywords:
Alzheimer’s disease;
cortex;
serum;
Aβ;
electro-acupuncture;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(10):875-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of eletro-acupuncture (EA)on the expression of Aβ1 -40 and Aβ1 -42 in cortex and serum of APP/PS1 transgenic mice with Alzheimer’s disease (AD),so as to explore the mechanism of EA therapy on AD according to the clearance of Aβacross blood brain barrier (BBB). Methods Twenty 6-month-old APP/PS1 transgenic mice were randomly divided into model control group and EA group,and ten more C57BL/6 wild type mice were assigned into the normal control group. After one and half months’EA treatment at the acupoint“Yǒngquán”(KI 1 )and“Bǎihuì”(GV 20), Aβ1 -42 expression in the cortex was observed by using immunohistochemistral staining,and content of Aβof cortex and serum were measure by ELISA method.Results In the model group,deposits of mature senile plaques were observed,which were weak in EA group .Compared with the model group,the ex-pression of Aβ1 -40 and Aβ1 -42 in EA group significantly decreased (P <0.01),both in cortex and in ser-um.Conclusion EA therapy can decrease the content of cortex and serum Aβ,especially on Aβ1 -42 which has neurotoxicity,which may be one of ways of EA treatment on AD.
