Complete genomic sequence analysis of the Guangzhou Norovirus strain SZ20133135
10.3760/cma.j.issn.1003-9279.2014.03.009
- VernacularTitle:诺如病毒广州株GZ20133135全基因组序列测定及分析
- Author:
Yi TIAN
1
;
Yinhui PEI
;
Miao JIN
;
Huaping XIE
;
Kena CHEN
;
Huiying LI
;
Zhaojun DUAN
Author Information
1. 河北联合大学基础医学院
- Keywords:
Noroviruses;
Genes;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(3):184-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain information on viral molecular structure and genome via full-length genomic analysis on the norovirus strain GZ20133135 isolated from Guangzhou.Methods Primers were designed according to the Sydney2012 full sequence.The full genome of the strain GZ20133135 was amplified by RT-PCR.The whole genome sequence was analyzed after cloned and sequenced.Results The genome of G Ⅱ-4 norovirus strain GZ20133135 consisted of 7566 bp,and it was revealed that there were three ORFs composites ORF1 (5100 bp),ORF2 (1623 bp),ORF3 (807 bp) respectively; ORF1 and ORF2 had 19 nt overlap.It was found by evolutionary comparative analysis that GZ20133135 genomic nucleotide sequences,compared with reference strains of G Ⅱ-4 Sydney2012 strains,the highest homology with a total length of homology was 99.07％.Phylogenetic analyses showed GZ20133135 belonged to G Ⅱ-4Sydney 2012 variant.Data of 541 amino acid analyses showed that Sydney 2012 variant strains of popular sites were aa294V or A or P→T,aa296S or T→S,aa297H or Q→R,aa298D or N or T→N,aa368T or N or S or A→E,aa372 N or S→D,aa393 N or D or S,aa394 T or G or S→T,aa395T or A→T,aa407 N or D→S,aa412T or D→N,aa413G or I→T.Conclusion Norovirus GZ20133135 belonged to the G Ⅱ-4 Sydney2012 variant.In This study,GZ20133135 full sequence information can be used not only as a full-length NoV variant sequence standard for future comparison studies,but also as useful material for the public health by enabling the diagnosis,vaccine development,and prediction of new emerging variants.
