Comparison of the DNA Preservation in Neutral-Buffered Formalin Fixed Paraffin-Embedded Tissue and in Non-Buffered Formalin Fixed Paraffin-Embedded Tissue.
- Author:
An Na SEO
1
;
Jae Hoon KIM
;
Dakeun LEE
;
Ji Yun JEONG
;
Ji Young PARK
Author Information
1. Department of Pathology, Kyungpook National University School of Medicine, Daegu, Korea. jyparkmd@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Tissue fixation;
Formaldehyde;
Tissue preservation;
Pathology, molecular;
DNA degradation
- MeSH:
DNA;
Electrophoresis, Microchip;
Fixatives;
Formaldehyde;
Microsatellite Instability;
Paraffin;
Pathology, Molecular;
Polymerase Chain Reaction;
Tissue Fixation;
Tissue Preservation
- From:Korean Journal of Pathology
2011;45(6):549-556
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The preservation of optimized DNA and its extraction from formalin-fixed, paraffin-embedded (FFPE) tissues are important issues. There has been some doubt over whether 10% neutral-buffered formalin is an ideal fixation solution for DNA preservation over non-buffered formalin, as conventionally recommended. In this study, the correlation between the efficiency of DNA extraction from FFPE tissues and buffered formalin was evaluated. METHODS: Several tissues with same conditions except fixatives were fixed in four different formalin solution groups and were routinely processed as paraffin-embedding protocols. DNAs were extracted from four different FFPE tissues that were stored for over 3 months and over 9 months. The quantity and quality of the DNAs were assessed with a NanoDrop ND-1000 spectrophotometer, and the polymerase chain reaction (PCR) amplification and degradation were analyzed via microchip electrophoresis. KRAS mutation analysis and microsatellite instability (BAT25) PCR were performed with each sample. RESULTS: The results showed no remarkable difference in the four groups. CONCLUSIONS: The study findings demonstrate that DNA preservation is fairly unaffected by a neutral buffer where there is short formalin manufacture period and an adequate formalin fixation time before embedding in paraffin.
