Use of indirect immunofluorescence microscopy in the diagnosis of AIDS patients with Pneumocystis pneumonia
10.3760/cma.j.issn.1003-9279.2014.02.021
- VernacularTitle:间接免疫荧光法在艾滋病合并肺孢子菌肺炎诊断中的应用
- Author:
Yan LIU
1
;
Songping ZHANG
;
Jinchuan SHI
;
Jianhua YU
;
Jianchun GUO
Author Information
1. 杭州市西溪医院(原杭州市第六人民医院)
- Keywords:
Acquired immune deficiency syndrome;
Pneumocystis carinii;
Fluorescent antibody techniqe,indirect
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(2):139-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect Pneumocystis carinii in sputum in patients with AIDS complicated with Pneumocystis pneumoniae (PCP)by using indirect immunofluorescence microscopy.Methods Indirect immunofluorescence microscopy was used to detect Pneumocystis carinii in sputum samples of 53 patients with AIDS complicated with suspected PCP,observed the clinical effects and outcomes of the patients.Results Of the 53 cases,30 cases were positive.The positive rate was 56.6％.Patients with positive CD4+ T lymphocyte count was in average 33.33 ± 42.99/μl,23 patients who were negative for PCP had a CD4+ T lymphocyte count of 104.13 ± 77.87/μ1.Conclusions The indirect immunofluorescence microscopy was specific rapid and sensitive method in detection of pneumocystis infection in HIV infected patients.
