Lentiviral vector expressing human IL15 in dendritic cells
10.3760/cma.j.issn.1003-9279.2014.02.019
- VernacularTitle:慢病毒介导白介素IL15在人树突状细胞中表达研究
- Author:
Ke XU
1
;
Xia FENG
;
Shuangqing YU
;
Yi ZENG
;
Shaohua XU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Interleukin-15;
Genes;
Dendritic cells
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(2):132-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate target gene IL15 expression by lentiviral vector in mammalian cells,we constructed a recombinant lentivirus expressing human interleukin 15 to be used as vaccine adjuvant or in gene immunotherapy.Methods The IL15 gene was cloned from human peripheral blood mononuclear cell (PBMC) and constructed into plasmid plvx-IL15-Ires-gfp.Recombinant lentivirus was produced in 293T cells and immature dendritic cells were infected in vitro.Results The target gene expression was detected,cell lines stably expressing IL15 were obtained by antibiotic screening.Immature dendritic cells can also be infected and express IL15.Conclusion We successfully constructed lentivirus vector that was able to express ILl5 in dendritic cells without inducing maturation,it could be used in gene immunotherapy or as a vaccine adjuvant.
