To investigate the AMPK-ULK1 signal transduction pathway in the scrapie 139A infected mice brain tissues

VernacularTitle:羊瘙痒因子139A感染的小鼠脑组织中AMPK-ULK1自噬调节通路的研究
Author: Xueyu FAN ¹ ; Hui WANG ; Yin XU ; Qixiang SHAO ; Xiaoping DONG ; Chan TIAN
Author Information

1. 江苏大学基础医学与医学技术学院免疫学系
Keywords: Protein Kinases,Scrapie; Autophagocytosis
From: Chinese Journal of Experimental and Clinical Virology 2014;28(2):126-128
CountryChina
Language:Chinese
Abstract: Objective To investigate the activation of the autophagy and the signal transduction pathway in the scrapie 139A infected mice brain tissues.Methods The Western Blot was used to evaluate the expression level of AMPK,ULK1,p-AMPK,P-ULKI and LC3 Ⅱ in the normal and scrapie 139A infected mice brain tissues.Results The expression levels of AMPK,ULK1 and their phosphorylated form p-AMPK (Thr172),p-ULK1 (Ser555) are markedly up-regulated in the brains of the mice infected with scrapie agent 139A.Furthermore,the expression of LC3 Ⅱ was also found increased in the brains of 139A infected mice,but not in normal mice.Conclusion AMPK-ULK1 pathway activated and contributed to autophagy in 139A infected mice brain.