Use of In-Fusion technology in cloning of the full-length genome of hepatitis B virus and phylogenetic analysis

VernacularTitle:应用In-Fusion技术构建HBV基因组全长重组质粒及序列分析
Author: Xun ZHANG ¹ ; Honglan ZHAO ; Liping SHEN ; Shengli BI
Author Information

1. 中国疾病预防控制中心病毒病预防控制所肝炎室
Keywords: Hepatitis B virus; Cloning,molecular; Genes
From: Chinese Journal of Experimental and Clinical Virology 2014;28(2):111-113
CountryChina
Language:Chinese
Abstract: Objective To investigate the use of In-Fusion technology in the sequencing of hepatitis B virus genome and analyzing the sequence of 23 strains of isolates.Methods Twenty-three strains of type B HBV genome were amplified by PCR from the serum collected in Zhejiang,China,and were inserted into the vector of pMD18T in the ways of In-Fusion technology,and the sequence of it was analyzed.Results The strains of the HBV isolates were types B and C,which were most common in China,and one isolate of YMDD mutation was found.Conclusion It was proven that the In-Fusion technology is an effective way to construct recombinant plasmid,avoiding self-connection of vector.The finding of this research provides a reference to the study of the genotype and evolution of hepatitis B virus in China.