Analysis of full-length hepatitis A virus genome isolated from a patient with acute hepatitis A in Beijing
10.3760/cma.j.issn.1003-9279.2014.02.010
- VernacularTitle:我国甲型肝炎病毒BJ73株全基因组测定与分析
- Author:
Hao WANG
1
;
Jingyuan CAO
;
Shengli BI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatitis A virus;
Genes;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(2):105-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the complete nucleotide sequence of hepatitis A virus (HAV) extracted from serum of an acute hepatitis A patient in 2007 (B J73) in Beijing.Methods The full genomic sequence was sequenced and subjected to the phylogenetic analysis using Bioedit 7.0,MEGA5 and HyPhy.Results The genome of BJ73 consisted of 7477 nucleotides,encoding a polyprotein of 2227 amino acids.Sequence comparison showed that B J73 shared the highest identities of 99.1％ for nucleotide and 99.7％ for amino acid with LU38.Amino acid change was not observed in reference to published neutralizing antigenic sites in VP3-VP1 regions.Phylogenetic analysis included the B J73 sequence in subgenotype IA,either considering different fragments or the full length nucleotide sequence; negative selection was found in coding regions.Conclusion This article provides new data on the distribution of genotype and virus evolution of HAV isolated from China.
