Increased peripheral mRNA levels of Trx-1 and Trx-2 associated with the progression of hepatitis B induced acute-on-chronic liver failure
10.3760/cma.j.issn.1003-9279.2014.02.009
- VernacularTitle:慢加急性肝衰竭患者外周血PBMC中Trx-1、Trx-2表达水平研究
- Author:
Yang YANG
1
;
Yuchen FAN
;
Zehua ZHAO
;
Xiangfen JI
;
Chengyun DOU
;
Jianjun ZHANG
;
Kai WANG
Author Information
1. 250012济南,山东大学齐鲁医院肝病科,山东大学肝病研究所
- Keywords:
Thioredoxin;
Oxidative stress;
Hepatitis B virus
- From:
Chinese Journal of Experimental and Clinical Virology
2014;28(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Trx-1 and Trx-2 in the progression of hepatitis B associated acute-on-chronic liver failure (ACHBLF).Methods We determined the mRNA levels of Trx1,Trx-2 in peripheral blood mononuclear cells (PBMC) from a cohort of 60 patients with ACHBLF,38 patients with chronic hepatitis B (CHB) and 26 healthy controls by fluorescence real-time quantiative PCR.Results We demonstrated that there were significantly higher expressions of Trx-1 and Trx-2 mRNA in patients with ACHBLF than those patients with CHB and healthy controls (P ＜ 0.05,respectively).Furthermore,the levels of Trx-1 and Trx-2 mRNA significantly correlated with total bilirubin (TBIL) levels in patients with ACHBLF (P ＜ 0.05,respectively).The relative expression of Trx-1 mRNA significantly correlated with model for end-stage liver disease (MELD) scores in patients with ACHBLF (P ＜ 0.05).Besides,Trx-1 and Trx-2 levels were higher in non-survivors with ACHBLF than survivors with ACHBLF (P ＜0.05,respectively).The relative expression of Trx-1 mRNA was predictive of 90-day mortality using Kaplan-Meier analysis (P ＜ 0.05),with a sensitivity of 75％ and a specificity of 64.29％.Conclusion Trx and associated oxidative stress may play an important role in the pathogenesis and progression of ACHBLF,the mRNA level of Trx-1 and Trx-2 may be associated with disease severity and poor prognosis of ACHBLF.
