Effect and mechanism of oldenlandia on Cxcl1 gene expression in macro-phages
10.3969/j.issn.1006-2157.2016.07.011
- VernacularTitle:白花蛇舌草对巨噬细胞Cxcl1基因表达的影响及机制的初步研究
- Author:
Xiaoning YUE
1
;
Yang SHI
;
Shizhu ZANG
Author Information
1. 大连医科大学生物技术系 辽宁116044
- Keywords:
Baihuashe Shecao(Hedyotis diffusa Villd.);
macrophage;
Cxcl1 gene;
Toll like receptor 4
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(7):580-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the immunoregulatory effect of Oldenlandia ( Hedyotis diffusa Villd.) , by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway , aiming to propose a new experimental and theoretical evidence for the use of oldenlandia . Methods Three common cytokines , such as IL-6 , MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution .Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression chan-ges of the cytokines.Results Oldenlandia solution could upregulate the expression of Cxcl 1, which might depend on the combination of TLR 4 and stigmasterol-beta-D-glucoside , trans-4-methoxycinnamyl alcohol , and asperulosidic acid .Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl 1 and function of macrophage .It can be inferred that oldenlandia could regu-late immunity , which could serve as the theoretical rationale for the development of new drugs in the labo-ratory.
