Effects of Bushen Yifei Xiaozheng Decoction on the expression of key mo-lecular genes and proteins in Caspase-12 signaling pathway in IPF rats
10.3969/j.issn.1006-2157.2016.07.010
- VernacularTitle:补肾益肺消癥方对特发性肺纤维化大鼠Caspase-12信号通路关键分子基因和蛋白表达的影响
- Author:
Yingxi YANG
1
;
Limin CHAI
;
Tiantian WU
;
Weihua GAO
;
Ziheng ZHU
;
Fang XU
;
Jiajun YU
;
Jun YAN
Author Information
1. 北京中医药大学东直门医院呼吸科 北京100700
- Keywords:
Bushen Yifei Xiaozheng Decoction ( kidney-tonifying lung-boosting stasis-resolving decoction );
idiopathic pulmonary fibrosis;
endoplasmic reticulum stress;
apoptosis pathways;
Caspase-12;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2016;39(7):575-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Bushen Yifei Xiaozheng Decoction ( kidney-tonifying lung-boosting stasis-resolving decoction ) on the expression of key genes and proteins in Casepase-12 signaling pathways in rats with idiopathic pulmonary fibrosis ( IPF ) .Methods IPF was induced with bleomycin in rats except for the normal group .Pirfenidone was used as positive control , while Bushen Yifei Xiaozheng Decoction ( TCM ) was administered during ( TCM preventive group ) and after ( TCM treatment group ) the model established and throughout the whole experiment ( TCM preventive and treat-ment group ) .Pathological change in the rat lungs was observed with Masson staining , meanwhile the ex-pression of molecular genes and proteins involved in Caspase-12 signaling pathways was detected by using Western blot and Real-time PCR.Results Masson staining revealed that the degree of fibrosis in TCM groups improved significantly and alveolar structure changed little . Protein and gene expression of Caspase-12 pathway in the model group was higher than those of normal group (P<0.05).In addition, the protein and gene expression of pirfenidone group and TCM groups were less than the model group ( P<0.05 or P<0.01), especially TCM preventive and treatment group .Conclusion Bushen Yifei Xi-aozheng Decoction could inhibit the process of pulmonary fibrosis via acting on the gene expression of Caspase-12 pathway and releasing the endoplasmic reticulum stress .
